Journal: iScience

Article Title: SAA1/TLR2 axis directs chemotactic migration of hepatic stellate cells responding to injury

doi: 10.1016/j.isci.2021.102483

Figure Lengend Snippet:

Article Snippet: AGER antibody , Proteintech , Cat#Ag23105; RRID:Addgene_80089.

Techniques: Marker, Polymer, Plasmid Preparation, Recombinant, Protease Inhibitor, Saline, Enzyme-linked Immunosorbent Assay, Activation Assay, Silver Staining, Mass Spectrometry, Bicinchoninic Acid Protein Assay, Transfection, Gene Expression, Reporter Assay, Staining, Over Expression, Software, Imaging, Live Cell Imaging, Microscopy

Journal: Signal Transduction and Targeted Therapy

Article Title: Revealing the crucial roles of suppressive immune microenvironment in cardiac myxoma progression

doi: 10.1038/s41392-024-01912-2

Figure Lengend Snippet: Macrophages promoted the proliferation of myxoma cells. a The number of significant ligand-receptor interactions between T cells and myxoma cells. b The number of significant ligand-receptor interactions between macrophage and myxoma cells. The direction of the arrow indicates the cell type expressing the ligand, while the direction the arrow points to represents the cell type expressing the receptor. c Barplot depicting the number of significant ligand-receptor interaction pairs (y-axis) between myxoma cells and macrophages (filled by different colors) in different pathways (x-axis). d Representative ligand-receptor pairs between myxoma cells and macrophages. The color indicates the strength of the ligand-receptor interactions, and the dot size represents the statistical significance of interactive molecular pairs. e The expression of genes is projected on the UMAP plot of macrophages. f The infographic (created with BioRender.com) summarizes predicted cell-cell interaction circuits in myxoma. g Incucyte proliferation assay of the co-culture system between myxoma cells and macrophages. h Immunofluorescence images show the proliferation of myxoma cells at different time points in the single culture and co-culture system. i Comparison of the number of myxoma cells in proliferative state at different time points in single culture and co-culture systems. j The secretion levels of some factors in a single culture and co-culture system are based on Elisa. The error bar indicates the standard error of the mean. k Comparison of myxoma cell proliferation status in the co-culture system after the addition of different inhibitors. HCH6-1, FPR1 antagonist. Crenolanib, PDGFR inhibitor. *P < 0.05; **P < 0.01; ***P < 0.001

Article Snippet: In the inhibitor-related experiments, we added DMSO, the FPR1 antagonist HCH6-1 (5 μM, Selleck, Cat. No. S0547), and the PDGFR inhibitor Crenolanib (50 nM, Selleck, Cat. No. S2730) to the co-culture system.

Techniques: Expressing, Proliferation Assay, Co-Culture Assay, Immunofluorescence, Comparison, Enzyme-linked Immunosorbent Assay